Technology
ARCHmap protocol
ARCHmap (Active CleaRing with CUBIC-LH, Hydrogel-embedding, and RI-matching with PuClear) is an efficient aqueous-based protocol for homogeneous clearing of the entire mouse body. This step-by-step protocol includes perfusion, postfixation, decalcification, delipidation, embedding, and RI-matching.
https://mesoanatomy.org/mesomouse/technology/ARCHmap
Blockface-VISoR system part list
Blockface-VISoR is a fluorescence microscope system for high-speed imaging of whole mouse specimens by serially sectioning and 3D scanning into the cut surface. The system integrates a sectioning module from a vibratome, and an imaging module based on the Volumetric Imaging with Synchronized on-the-fly-scan and Readout (VISoR) technology (Wang, H., et al., Nat. Sci. Rev., 2019; and Xu, F., et al., Nat. Biotechnol., 2021).
https://mesoanatomy.org/mesomouse/technology/blockface-VISoR-partlist
MesoMouse Browser
High-resolution 3D structure of the whole mouse body was imaged using a custom high-speed blockface volumetric imaging system (blockface-VISoR) along with an optimized workflow of whole-body clearing and embedding (ARCHmap). The mouse body, measuring approximately 4 × 2 × 8 cm3, was sectioned at 400 μm increments and imaged 600 μm into the blockface to obtain a series of 3D images at a voxel resolution of 1 × 1 × 2.5 μm3 for later reconstruction. The sectioning-imaging process for the whole body takes about 40 hours. The raw image data collected for the whole mouse exceeds 70 terabytes per channel.