| Laser box |
Coherent Laser Box OBIS |
1 |
Optional. A convenient laser enclosure Integrated with five bays for OBIS lasers, heat sink, cooling, power and control interface. |
| LASER 405nm |
Coherent OBIS 405-100 LX |
1 |
You choice of excitation light sources. KHz modulation is mandatory. |
| LASER 488nm |
Coherent OBIS 488-100 LS |
1 |
|
| LASER 552nm |
Coherent OBIS 552-100 LS |
1 |
|
| LASER 640nm |
Coherent OBIS 640-100LX |
1 |
|
| Filter/dichroic |
Semrock LM01-427-12.5 |
1 |
Dichroic filter for combining 405 laser into longer wave-length laser beam. |
| Filter/dichroic |
Semrock LM01-503-12.5 |
1 |
Dichroic filter for combining 488 laser into longer wave-length laser beam |
| Filter/dichroic |
Semrock LM01-613-12.5 |
1 |
Dichroic filter for combining 640 and 552 laser beams |
| Mirror |
Thorlabs PF05-03-P01 |
13 |
Folding mirrors |
| Mirror mount |
Thorlabs KM05/M |
13 |
Mirror mounts for PF05-03-P01 |
| Mirror |
Thorlabs PF-10-03-P01 |
1 |
Folding mirror |
| Mirror mount |
Thorlabs KM100 |
1 |
Mirror mounts for PF10-03-P01 |
| Galvanometer |
Thorlabs GVS001 |
1 |
This device works at 250Hz with scanning range ±3.5°, which is not charted in manufactory specification. |
| Scan lens/Tube lens |
Thorlabs AC254-100-A-ML |
2 |
1:1 relay of scanning mirror to the back aperture of the excitation objective. |
| Objective/excitation |
Olympus UMPLFLN10XW |
1 |
Objective lens for excitation. |
| Objective/imaging |
Olympus UMPLFLN10XW |
1 |
Objective lens for imaging. Switchable with UMPLFLN20XW for higher resolution and, of cause, smaller field of view. UMPLFLN40W is also tested. |
| Camera |
Hamamatsu C13440-20CU |
2 |
|
| Tube lens |
Olympus U-TLU |
2 |
|
| C-0.63x adaptor |
Olympus U-TV0.63XC |
2 |
For larger field of view. |
| Motorized Filter Switcher |
Bitelligen B-V-EF1034 |
1 |
Optional. The motorized filter switcher offers 3 independent motorized rails, one 3 position rail for dichroic filters and two 4-position rails for emission filters. |
| Filter/emission |
Chroma ET455/50m |
1 |
|
| Filter/emission |
Chroma ET520/40m |
2 |
|
| Filter/emission |
Chroma ET600/50m |
2 |
|
| Filter/emission |
Chroma ET665lp |
1 |
|
| Filter/dichroic |
Chroma ZT488rdc |
1 |
|
| Filter/dichroic |
Chroma ZT561rdc |
1 |
|
| Filter/dichroic |
Chroma ZT640rdc |
1 |
|
| Motorized translation X |
Thorlabs LTS150C/M |
1 |
Position and translation of sample in x direction. Translating continuously at constant speed during imaging acquisition |
| Motorized translation Y |
Thorlabs LTS300C/M |
1 |
Position and translation of sample in y direction. Chang the field of view in y direction after continuously imaging of sample in one column along x direction. Also as part of whole vibratome, i.e., translating the sample against vibrational blade for sectioning. |
| Motorized translation Z |
Zaber X-VSR20A-KX13A |
1 |
Position of whole sample stage in z direction. |
| Vibratome |
Bitelligen B-B-DC42c |
1 |
The sectioning mechanism of the system that removes specified amount of sample after imaging. A replacement part for Bitelligen vibratome and is available to order directly from Bitelligen. (http://en.bitelligen.com/) |
| Sample module |
Bitelligen |
1 |
A custom designed unit including x, y, z motorized stages, motorized sample dispenser with integrated imaging chamber, and vibratome. Bitelligen is providing this unit as a module for order. (http://en.bitelligen.com/) |
| Beam combining module |
Bitelligen |
1 |
A structural module that holds beam combining filters and folding mirrors. Bitelligen is providing this unit as a module for order. (http://en.bitelligen.com/) |
| Beam scanning module |
Bitelligen |
1 |
A custom designed module that holds beam scanning galvo mirror assembly, relay lenses and folding mirrors. Bitelligen is providing this unit as a module for order. (http://en.bitelligen.com/) |
| V shape imaging head |
Bitelligen |
1 |
A custom designed module that holds both excitation objective and imaging objective in V shape configuration. Also features a 45° light deviator that makes up/down stream of optical train horizonal. Bitelligen is providing this unit as a module for order. (http://en.bitelligen.com/) |
| Duel channel imager |
Bitelligen |
1 |
A structural module that holds two copies of tube lens-camera units, and a motorized filter switcher with the dichroic and emission filters. Bitelligen is providing this unit as a module for order. (http://en.bitelligen.com/) |
| Frame assembly |
Bitelligen |
|
A whole set of parts to set the frame for the system, holds all the major parts and/or modules introduced. Bitelligen is providing this assembly for order. (http://en.bitelligen.com/) |
| Computer |
Generic |
1 |
Customized computer for image acquisition and system control. Recommended: minimum of 3GHz 24 Core 48 Thread Processor, 192G DDR4 high speed low latency memory, 32T NVMe SSD, and enough additional space for backup. |
| DAQ board |
National Instruments PCIE-6374 |
1 |
One mandatory AO and 24 programmable DIO for controlling of scanner, lasers, cameras, and stages. |
| DAQ cable |
National Instruments SHC68-68-EPM |
1 |
Accessory for DAQ board |
| DAQ connector |
National Instruments SCB-68 |
1 |
Accessory for DAQ board |
| Software for system control |
Bitelligen fastLSM |
|
A modified version of fastLSM from Bitelligen, the system control and imaging software originally used in Bitelligen high speed imagers. (http://en.bitelligen.com/) |